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Published by Universitas Negeri Padang

ISSN : 2614669X EISSN : 2579308X DOI : -

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

Bioscience ISSN 2579-308X (Electronic) ISSN: 2614-669X (Print) is peer-reviewed journal and scientific journal publish by Universitas Negeri Padang. The aim of this journal is to publish articles dedicated to all aspects of the latest outstanding developments in the field of biology. Scope of this journal is ;Environmental Biology; Genetics and Biotechnology; Biology of Function; Systematics, Structure and Development.

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Articles 9 Documents

Search results for , issue "Vol 2, No 2 (2018): Biology" : 9 Documents clear

Antioxidant Activity, Antibacterial Activity, Water Content, and Ash Content In Baduy Honey Adri Nora; Anjas Wilapangga; Titta Novianti
Bioscience Vol 2, No 2 (2018): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Indonesia as a tropical country has many different types of honey. Honey was known to have many advantages especially in health promotion as antioxidant and antibacterial. Baduy honey is one of Indonesian forest honey which its nutritional value, antioxidant, and antibacterial activity have not been investigated yet. The purpose of this study to determine antioxidant activity, antibacterial, water content, and also ash content of baduy honey. The research method are DPPH radical scavenging-activity for antioxidant, microplate resazurine assay for antibacterial, qualitative photochemistry analysis, and gravimetric analysis for measuring water and ash content. The result showed that black and yellow baduy honey has water content 18,64% dan 19,32% respectively and ash content 0,32% dan 0,04% respectively. Baduy honey has also containing flavonoid, terpenoid, and alkaloid compounds. Black Baduy honey has higher antioxidant activity than Yellow Baduy honey in which EC 50 values are 1000,79 μg/mL and 1475,28 μg/mL respectively. However, Baduy honey did not show any antibacterial activity againts Escheria coli dan Staphlococcus aureus because of MIC value for both type of honey are tremendously large with 500000 μg/mL.

Morphology Characteristics of Rattan (Calamus SP.) Tourism Forest In Bariat The South Sorong Regency Irnawati Irnawati; Lona Nanlohy
Bioscience Vol 2, No 2 (2018): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Rattan grows naturally inbeaches and mountains. Ecologically, it could grow rapidly in various places especially with high humidity areas such as riverside. The research conducted to identify and discover the morphology characteristics of botanical rattans which are being used by the local communities in Bariat natural Park Forest.Theresults of this research can be used for the cultivation and conservation of rattan (calamus SP.). The methodology of research is descriptive which is conducted with field visits. The first samples were collected randoml, however, the second and third sample was collected systematically. These collection method to determine the distribution of rattan growth. Five types of rattan were found in the research area which is divided into three classifications. Two of them are classified as Calamus, the other two are Daemonorops and the last one is Korthalsia. Turning into the way of the rattan grow in Bariat, four of them grow as clumping plants while the other one is single trunk (solitary). In Bariat, the rattan has low diversity but high quantity. It is potentially to be used as a source of biodiversity which has high economy value for the local community

Isolation and Characterization of Lactic Acid Bacteria (Lactobacillus sp) from Tomato (Solanum lycopersicum) Resti Fevria; Indra Hartanto
Bioscience Vol 2, No 2 (2018): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Prebiotics are good coumpounds used in the growth of lactic acid bacteria (LAB) in digestion, prebiotics come from vegetables, fruits and seeds. Tomatoes are thought to be one of the natural media of bacteria because tomatoes contain various chemical compositions that are needed as a substrate for LAB. The purpose of this study is to isolate and determine the type of LAB produced microscopically. The ingredients used are tomatoes obtained from Padang Panjang market, with Merck NA media, oxoid MRSa, 0,9% NaCl, and crystal violet paint. Isolation LAB from tomatoes done in two ways, the first with plant tomato tissue directly into the NA medium and the second with fermentation tomato and then plant the tomato into the MRSa medium with streak plate methods. The isolates obtained were identified microscopically using a microscope with gram staining method. From the research that has been done, the following result are obtained : (1) Plant tomatoes tissue direcly into NA medium and gram staining, there were 1 colonies of gram positive bacteria with bacil cell form. (2). Fermentation tomatoes from the results, there were 2 colonies gram positive bacteria with bacil cell form. We can identify this colonies as Lactobacillius bulgaricus.

Quantity of Germination Seed Response to Combustion Duration and Light Treatments in Jabon (Anthocephalus cadamba (Roxb.) Miq.) Eliza Gusmira; Vauzia Vauzia
Bioscience Vol 2, No 2 (2018): Biology
Publisher : UNIVERSITAS NEGERI PADANG

This research was aims to determine the response of jabon seed germination toward duration of combustion and light. This research was conducted from January to June 2018 at the Biology Laboratory and FMIPA UNP greenhouse. The design used was RAL with 2 factors. The first factor is the duration of combustion and the second factor is the light treatment. Based on the design obtained 10 treatment combinations and each treatment was repeated 3 times so that there are 30 units of experiment. Parameter observed is the number of seeds germination. The results showed that light treatment, combustion duration treatment, and interaction between combustion duration and light give good effect on the number of seeds germination. The seeds with the combustion treatment for 15 (A2) minutes had the highest number of sprouts that was 342.83 sprouts per 0.2 gram of jabon seeds. For light factor, many seeds germinate on the shelter treatment (B2) of 349.59 seeds per 0.2 gram of seed. While the combination of treatments showed that the best number of seeds germinated was in the combustion treatment 12.5 minutes with germination in the sheltered place (A1B2) that produced 564.67 sprouts per 0.2 gram of seed.

The influence of carbon sources on the production of siderophores from the fluorescent pseudomonad bacteria Ilham Pratama; Linda Advinda; Mades Fifendy
Bioscience Vol 2, No 2 (2018): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Abstract.Pseudomonad fluorescent is one of the rhizobacteria groups that could potentially be developed as a crop endurance inducer. Several species of fluorescent pseudomonad are able to produce siderophores. Siderophore is an antimicrobial organic compound that plays a role in biological control of plant diseases. This study aims to determine the best carbon source for the production of siderophores from the fluorescent pseudomonad isolates PfCas3 and PfLAHp2. The carbon sources are fructose, glucose, and glycerol. Detection of siderophores was measured using a spectrophotometer at a wavelength of 410 nm. The results showed that the best growth medium for producing siderophores was KB + glucose medium for both PfCas3 and PfLAHp2 isolates. The best combination was the use of PfCas3 isolate with the addition of carbon glucose source which resulted in the production of siderophores of 1.574. Keywords: Fluorescent pseudomonad, carbon sources, siderophore

Candida antarctica Lipase B Synthetic Gene: A Bioinformatics Analysis Febriana Dwi Wahyuni; seprianto Seprianto
Bioscience Vol 2, No 2 (2018): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Enzyme exploration is important to support the development of biotechnology. To facilitate microbial discovery, it can be done synthetically using bioinformatics methods. Candida antarctica lipase B (CALB) is one of enzyme derived from microorganisms and has been applied as a biocatalyst in several industry. The methods used in this study are the analysis of gene structure using NCBI, analysis of protein CALB sequence using Uniprot, analysis of 3D protein structure using Swiss model, analysis primary design using primer3 and analysis of restriction sites using snapgene. The construction of synthetic calB gene obtained based on the results of the CalB gene sequence in geneBank using NCBI with access number Z30645.1. CalB gene wildtype is modified by adding several restriction enzyme (XhoI, XbaI, ClaI, and BglI), 6 Histags, and 2x stops codon and produce 1083 base pairs. CALB protein has 342 amino acids. Based on 3D structure, CALB protein has three molecules in common with one homotrimer ring that will encircle the double helix DNA. Primers used for calB gene amplification are CALB forward 5'-TCCCCAGTATCAGGTCCAAG-3 ' and CALB reverse 5'-GACACCTGAGGCTGAACGAT-3'.

The Thermophilic Bacterial Growth Curve Irdawati Irdawati; Ilsa Septia Putri; Syamsuardi Syamsuardi; Anthoni Agustien; Yetria Rilda
Bioscience Vol 2, No 2 (2018): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Thermophilic bacteria are bacteria that can produce thermostable enzymes and used in various industries. Thermostable enzymes that can be produced by thermophilic microorganisms one of them is xylanase enzyme. Xylanase produced by microbes has optimum temperature characteristics and more diverse optimum pH on various substrates, which will affect the activity of the resulting xylanase enzyme. The influence of temperature greatly determines the activity of the enzyme at the time of catalyzing a reaction. At optimum pH conditions, the enzyme has an active side conformation that is substrate-like so that it can form a complex of appropriate enzymes and produce the product to its full potential. One of the thermophilic bacterial habitats of hot springs in West Sumatera is the Aro Sapan River hot spring located in Koto Parik Gadang Subdistrict in Ateh, South Solok District. The Saw Aro River hot spring has a temperature of 75 ° C and is pH 8 or alkaline. The aim to this research was to know the profile of the growth of thermophilic bacteria. This research is a descriptive method. The data obtained were analyzed descriptively. The conclusion is the best activity of xylanase thermophilic bacteria was at the sixth time incubation.

The Effect of Composting Azolla Compost Fertilizer and Humic Material on CO2 Gas Production in Sand Land Febrianti Rosalina; Muhammad Syahrul Kahar
Bioscience Vol 2, No 2 (2018): Biology
Publisher : UNIVERSITAS NEGERI PADANG

his study was an experimental study which aimed to determine the effect of Compost Azolla Fertilizer (KA) and the addition of humic material (H) on the sand soil to the levels of CO2 produced in the respiration process of soil microorganisms (incubation soil conditions). The design used is a Completely Randomized Design (CRD) consisting of 9 treatments, namely 300 kg ha-1 urea (U), 4 tons ha-1 KA (T1), 15 liters ha-1 H (T2), 2 tons ha-1 KA + 15 liters ha-1 H (T3), 4 tons ha-1 KA + 15 liters ha-1 H (T4), 6 tons ha-1 KA + 15 liters ha-1 H (T5), 2 tons ha-1 KA + 30 liters ha-1 H (T6), 4 tons ha-1 KA + 30 liters ha-1 H (T7), 6 tons ha-1 KA + 30 liters ha-1 H (T8) and 1 control (K ) without the addition of train and H with 3 replications. Measurement of carbon dioxide (CO2) levels from each sample using gas chromatography equipped with TCD (Thermal Conductivity Detector). The statistical data analysis used was the F test, whereas to see the difference in the effect of each treatment, the BNT test was used. The results showed that Azolla compost fertilizer and humic material on sand soil had an effect on the level of CO2 gas production produced in the soil microorganisms respiration process, where the concentration of Azolla compost fertilizer and humic material which most affected the increase of CO2 gas production in sand soil was treated with the highest concentration of 6 tons ha-1 Azolla + 30 liter compost fertilizer ha-1 H (T8).

The influence of carbon sources on the production of siderophores from the fluorescent pseudomonad bacteria Ansel Nastika; V Violita; Irma Laelani Eka Putri
Bioscience Vol 2, No 2 (2018): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Land kangkung (Ipomoea reptans Poir.) is one of the types of kangkung plants that are quite easy to be cultivated but because of the narrowness of urban agriculture land, it is difficult for the people to cultivate kangkung. Now there are one alternatives to exploit narrow land as an effort to develop organic farming, that is by farming in a hydroponic way. This study aims to determine the effect of liquid organic fertilizers made from Sargassum sp. on the growth of land kangkung (Ipomoea reptans Poir.) with hydroponic technique. This research is an experimental research conducted in December 2017 until April 2018, housed in research laboratory and home wire Biology FMIPA UNP. This study used Completely Randomized Design (RAL) 6 treatments and 4 replications. The main observation parameters include Plant height (cm), Number of Leaves, Leaf Area (cm2), Plant Wet Weight (g) and Dry Weight Plant (g). The data were analyzed using Analysis of Variance (ANOVA) test and DNMRT further test at 5% level. The results showed that liquid organic fertilizer made from Sargassum sp. had no signifficant effect on plant height, surface area, number of leaves, wet weight and dry weight of land kangkung (Ipomoea reptans Poir.)

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